Re: [DIYbio] Re: Non-Platinum Electrophoresis Electrodes That Don't Degrade

Interesting idea Conner!  I had no idea spark plugs had Pt.  Also, good point Nathan about the point source causing problematic distortion in a 2D gel.  I wonder if a decent electrical field could be created with an evenly spaced array of spark plugs (or small lengths of Pt wire).  Somebody fire up some E field modeling* :)

-Brian

* COMSOL or ANSYS might be fairly turnkey... or, for the brave, Mathematica/Matlab/NumPy should do it.  I haven't even Googled it (let alone Google Scholar), so forgive me if prior work on the electrode array front is laying in plain sight.

On Monday, May 27, 2013 at 4:57:24 PM UTC-4, Nathan McCorkle wrote:

A point electrical source might drag all the DNA to the center of the gel

On May 27, 2013 6:46 AM, "Conner Berthold" <cmb4...@gmail.com> wrote:

I had an idea a few days ago about using platinum spark plugs as the electrodes. One could be placed at each end and the center used for the electrical in/outputs. I hope to be building a few boxes soon and would just thread holes for them. Another plus would be that they could be changed out easy if they ever corroded.

Any ideas?

-Conner

On Sunday, May 26, 2013 2:29:19 PM UTC-4, Josiah Zayner wrote:

I have been testing out a bunch of different metals and they either oxidize to death on the minutes timescale or in the case of stainless steel turn the buffer orange/brown.

Platinum wire is pretty expensive ~$1.00 cm^-1. Has anyone had luck with a cheaper metal at 100V or greater with TAE?

I have tried:

Nickel

Stainless Steel 430

Tinned Copper

Thinking of trying Titanium or Nickel Titanium (<= $4 meter^-1). Anyone ever tried these?

Thanks,

     Josiah Zayner

http://DoItOurselfScience.blogspot.com 

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