I've never tried SliCE but I heard reports of increased mutation rate. Is that possible?
As in the article shared by koeng, cloning should be simple, DH5 alpha should have enough recombinogenic activity/ligase to splice your fragments, if used for the production of the extract.
Second question: can you make this extracts and sell/ship them?
Fede
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Re: [DIYbio] Re: The ODIN Needs Your Help - T4 DNA Ligase Testing - Free Ligase
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