The old machine I have (which isn't even serviced by the company anymore) has a really bright and hot halogen lamp in the middle of an open chamber shaped like a flying-saucer. There are cradles you put either capillary glass tubes in, or a cradle that holds normal strip PCR tubes, and suspends them in the air. When the machine ramps up, really fast hot air circulates around the chamber and evenly heats the tubes, then when it needs to cool a flap at the back opens up and lets hot air out, and opens and shuts rapidly to keep the temperature steady. It definitely ramps and cools faster than a normal PCR machine.
There is a paper here which are the tubes one can get but which I've never tried.
That machine has had tube caps pop open and the reaction effectively ruined, but if the caps are on tight, they normally don't pop off. It's basically a highly-engineered professional "lightbulb PCR" machine.
On Mon, Feb 8, 2016 at 11:58 AM, Sebastian S Cocioba <scocioba@gmail.com> wrote:
Yes the caps can and will pop around the 95c region if no lid secures them in place. The higher end tubes tend to hold for longer. Another reason why some heated lids clamp down hard or have a screw-based latching system. If the whole tube is heated uniformly then it should not matter but if there is a thermal gradient the condensate which wont fall back down due to capillary action on the lid and walls will not be exposed to the thermal cycling thus waste the reaction.
Sebastian S. CociobaCEO & FounderNew York Botanics, LLCBlog: ATinyGreenCell.comOn 02/08/2016 10:21 AM, Sebastian S Cocioba wrote:Yeah the mineral oil is layered on top of reaction mix to keep the liquid below it from evaporating in the absence of a heated Lid.
So, the lid stays hotter at all times, so no vapor can condense on it? The vials will have caps, so it is close to a sealed system where some easy thermodynamics rules can be applied. Evaporation will only be local movement and condensing again as
pressure increases a little, and diffusion moves vapor to a cold surface. Is the vapor pressure ever enough to pop caps off?
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