Yes the caps can and will pop around the 95c region if no lid secures them in place. The higher end tubes tend to hold for longer. Another reason why some heated lids clamp down hard or have a screw-based latching system. If the whole tube is heated uniformly then it should not matter but if there is a thermal gradient the condensate which wont fall back down due to capillary action on the lid and walls will not be exposed to the thermal cycling thus waste the reaction.
Sebastian S. Cocioba
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com
On 02/08/2016 10:21 AM, Sebastian S Cocioba wrote:Yeah the mineral oil is layered on top of reaction mix to keep the liquid below it from evaporating in the absence of a heated Lid.
So, the lid stays hotter at all times, so no vapor can condense on it? The vials will have caps, so it is close to a sealed system where some easy thermodynamics rules can be applied. Evaporation will only be local movement and condensing again as
pressure increases a little, and diffusion moves vapor to a cold surface. Is the vapor pressure ever enough to pop caps off?
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