[DIYbio] Re: PCR trouble

16s worked and my plasmid looked good on the gel. Estimating from the ladder my template was around .06 nanograms in the PCR reaction. But Phusion, Phusion + DMSO, Q5, and Q5 + DMSO all failed to amplify.

A friend is sending me some primers that he's used successfully on pVIB before, I'll try it out and see how it goes. He doesn't remember exactly what they amplify but maybe I can figure it out and use them to figure out what's going on with my reaction.

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