Found a primer sequence here...
-- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016539/
(Table 3)
Forward primer = AARTACACATACCARAACAAAGTGGT
Reverse primer = TCCRCTCCCYCTYTGGTCTTG
Here's a good site for aligning virus sequence...
http://www.viprbrc.org/brc/home.spg?decorator=flavi_zika
So you'd need an extraction kit like QIAamp Viral RNA Mini Kit, a one-step RT-PCR kit, the primers, and a gel or spec for measuring amplification. A bit expensive for a one-off test, but you can probably get samples if you shop around.
On Monday, March 28, 2016 at 2:53:34 AM UTC-7, Bryan Daniels wrote:
On Monday, March 28, 2016 at 2:53:34 AM UTC-7, Bryan Daniels wrote:
I am just back from Spring Break in Puerto Rico and have come down with a 103 F fever, sore eyes etc. CDC has classified PR as high risk Zika zone. Most likely I have influenza, but on the off-chance it is Zika, I thought it might be an interesting DIY bio project. (I am male and past pregnancy stages in our family. . .)
I have poked around on-line and there appear to be PCR based diagnostic tests, so there are clearly primers available. Also found this article: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285245/
I'm wondering if anyone in DIY bio community has tried this test themselves (on the cheap). As I'm not feeling too well right now, so I am also interested in how to preserve samples (and what type of samples) for later use. Any straight-forward protocols, would be much appreciated.
I have a reasonably well equipped basic lab, but only ordinary PCR capabilities, I.e., no real-time OCR.
Thanks in advance.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/408167a1-9e88-46c9-8a27-dc3fd5163fe1%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment