Re: [DIYbio] Re: PCR trouble

23.8 nmole was diluted in 238 uL of water
Then I diluted 5uL of that in 50uL of water

Then I used 2.5uL in my 25uL reaction.

I've also done different dilutions with the same but all 0.001 nmole per uL or less.

I'm also getting very bright bands even with that diluted 1000 more times.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/8f1fd056-15a2-44cd-8933-088900bfd08c%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.