Just to make sure - that thing doesn't have a heated lid, right?
You're using mineral oil?
It's not often the machine is the problem, but always good to check.
You're following good practice in setting up your PCR reactions and keeping things on ice until you're about to start?
On Sat, Mar 5, 2016 at 10:45 PM, David Ishee <midgardkennels@gmail.com> wrote:
I think the main thing controlling the current is the cheapness of the batteries and the amount of electrode surface in contact with the buffer. It actually stays cool even with 150v, and I get maybe 10 gels out of a bank of batteries.
This is my PCR machine
https://scontent-atl3-1.xx.fbcdn.net/hphotos-xpt1/t31.0-8/fr/cp0/e15/q65/12622378_1540136766299061_5737772239883873713_o.jpg?efg=eyJpIjoidCJ9
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