That's an old guide but there are many out there like it. You could drop your annealing even lower. To,bad you don't have a second set of primers that you could use as a control on pVIB
On Mar 5, 2016 7:51 PM, "Dakota Hamill" <dkotes@gmail.com> wrote:
-- I havn't used it in PCR in a while. I'm sure NEB, Google, or someone else on here could give you a more specific concentration. It can help with tough PCR.On Sat, Mar 5, 2016 at 7:42 PM, David Ishee <midgardkennels@gmail.com> wrote:What does DMSO do for it I have some. How much would I use?
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