Generally receptors are too low in expression to isolate directly in tissue. Make recombinant clone for over expression in E. coli, yeast, Pichia, baculovirus / insect cells etc.
Then you can purify & do crystal. Otherwise you can just map imputed amino acid sequence over existing crystal of homologous protein (I.e. in silico)
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On Mar 6, 2016 5:07 PM, "Mega [Andreas Stuermer]" <masterstorm123@gmail.com> wrote:
Hi everybody,
-- I was wondering whether it is possible to characterize a specific receptor from an animal species.The human homologue of the receptor is known.
So what would be needed is extract the receptor from the tissue, and analyze the structure. Is there a DIY way to do it? Is there anyone working on a similar project? How much would the equipment cost roughly?
Would be very grateful for any keywords you might know!
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