I make em every three weeks. Store in -20C in TSS buffer. I make how much I normally use in two weeks plus minus a bit. It isn't a big deal. For super tricky ligations I make em fresh day of. Never missed a clone. How many colonies do you really need, though?
Sebastian S. Cocioba
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com
Hi all,
Any ideas on how to store moderate competency cells without a -80c freezer? I've read the cfu/ug goes down dramatically if stored at only -20c? How are you guys doing it?
Thanks,
-Will
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