Re: [DIYbio] Re: DIY electroporator

I'd be interested in testing prototypes. I really like my current yeast transformation protocol because I find it easy enough, but I'd be very willing to test it out with E coli.


On Sunday, May 1, 2016 at 3:56:48 AM UTC, John Griessen wrote:
On 04/30/2016 09:29 AM, William Beeson wrote:
> I will test transformations on E.coli and Neurospora crassa.  I've had interest in a low cost electroporator for months.  I think
> the path to validation would be to show effectiveness with E. coli first (a much easier organism to transform).  Then transition
> to fungal cells which have much lower transformation efficiency.  If you have the hardware expertise to build a device with
> pre-programmed settings (say an E. coli and a fungal cells setting) I can do the lab work and prepare a data package for the product.
>
> -Will
>
> On Saturday, April 30, 2016 at 9:54:35 AM UTC-4, John Griessen wrote:
>
>     On 04/29/2016 07:33 PM, William Beeson wrote:
>      > If anyone has ideas for how to get this to work well please post or share the ideas.  The amount of electrical engineering
>      > involved in this is beyond my skill level.

How fast are you wanting this?  I'm in the middle of another project.
Anyone else interested in early testing of prototypes?  These will be "junk prototypes".
Similar to DIY art assemblages, but a little neater, and documented so a product
could be made from them by a few revision iterations.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/1c706efb-fbe9-463f-8c94-2ec3150d1d2c%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment