On May 15, 2016 1:21 AM, "Jake" <jakestew@mail.com> wrote:
>
> Way cheaper to just order them. You can get old oligo synthesizers for next to nothing if you look hard enough. But you can't buy the consumables for a reasonable price unless you want to start a company and do it full time. Even then you'd be using old tech and couldn't compete in the market.
Reagents and waste stream disposal costs are much too great to deal with unless you're going to utilize such a machine very often and have a steady stream of money coming in to pay for things. Acetonitrile, very-very-dry gas for flushing the reaction equipment and controlling valves (water looks like the hydroxyl on a nucleotide and terminates chain synthesis), and I haven't even got to the activation acid or a few other required items.
How I know? I have a synthesizer sitting unused for 2 years because I don't want to deal with all that annoyance (when all I want to do it is design and validation of genetic constructs).
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