Why doesn't someone just put a GFP plasmid with kanamycin selection into the yogurt. Might need a different ORI but it would be worth the effort to just do it and not speculate. If it does not fold or the chromophore does not oxidize then so be it but I think its worth attempting nonetheless. The low pH might be an issue so go for a more pH stable fluorescent protein like RFP.
Sebastian S. Cocioba
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com
Here's an interesting thought I've been toying with:--One of the classical yogurt making bacteria is Streptococcus thermophilus, a close relative of S. pyogenes, which is the source of the canonical Cas9 gene.Turns out that S. thermophilus has a native Cas9 system. So in theory, all we need to do genome editing in S. thermophilus would be to provide it with an artificial CRISPR casette!Completely bypasses any IP issues around Cas9 as well... :-)Patrik
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/2795a726-6fe1-448f-a9f7-e19df55ab6af%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment