[DIYbio] Re: A hypothetical protocol for DIYBIO DNA synthesis

I can't honestly say I've ever noticed that I've never tried to purify a thermophilic enzyme. I'm assuming you use heat to break down other enzymes and use some basic size based chromatography to seperate out from unfolded peptides etc?

So your consern is that if you needed a custom enzyme people wouldn't be bothered to do it? Perhaps. But you'd only need to do that if you couldn't buy cleanamp in which case you might not be able to buy lots of enzymes like tdt etc.

I wanted to put some thought into how people could make a dna synthesis technique work with minamal cooperation from the existing biotech industry. I don't know how the Americans feel but here in the uk the goverment and the established biotech industry doesn't seem to see much potential in the diybio movement but does see the potential for harm. The diybio movement has been compared to the coding movement that's gave birth to the raspberry pi. If so DNA would be the code and dna synthasis the compiler. Imagin if every raspberry pi came with no compiler. Instead you have to send your source code off to a company that might compile it, if they approve of it, for a charge. That situation would be unthinkable but it's more or less where we are with diybio.

So as I said I wanted to look into how you might make dna assuming virtually no cooperation from industry. But if you can order off the shelf enzymes I don't see why you wouldn't. Unless you were making enough for economies of scale to apply., I've been thinking about it and making it using bogstandard DNAse II and endonucliases might even work better.

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