Just that thermophilic enzymes are easier to purify.
-- Nickel columns are pretty easy as well, but the issue is is that the enzymes are likely patented, so you couldn't actively sell them. Of course, people could always purify it themselves, but that takes a lot of work. Even though I have his tagged most of the enzymes I commonly work with, I don't purify them, because it's too much work. I fear people would think it's too much work to get their own synthesis enzymes as well.
-Koeng
On Monday, June 13, 2016 at 3:42:47 PM UTC, CodeWarrior wrote:
On Monday, June 13, 2016 at 3:42:47 PM UTC, CodeWarrior wrote:
I don't understand why the enzymes would need to be thermophilic except for the assembly PCR step which you can use off the shelf enzymes for. Personally the only enzyme purification I've done is with hist tags and nickel ion chromatographic columns. It's scalable in the sense that you can always get a bigger column or a larger broth tank for culturing bacteria.
On Saturday, June 11, 2016 at 6:53:41 PM UTC+1, Koeng wrote:I'm not a chemist by any standard, but I do occasionally purify my own enzymes. Is there any solution for mass production of the enzyme itself? Purifying the enzyme itself may be more work than the system itself (not thermophilic), and modified versions of the enzyme required would likely be patented, preventing a large scale distribution network (other than for the people who patented it, and they likely won't be too DIY friendly). I'm not qualified to ask about most of the chemistry, but this is my first impression of one of the difficulties.-Koeng
On Friday, June 10, 2016 at 12:43:50 AM UTC, CodeWarrior wrote:My apologies I've said something stupid. That should be P3O10H5 not P3O8H5.
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