[DIYbio] Re: A hypothetical protocol for DIYBIO DNA synthesis

Well, what if we protect the 3'-O enzymatically? I didn't study in depth, but it seens promising. There are some papers on the field, like this one:

http://sci-hub.cc/10.1007/978-3-7091-6310-8_4

(I've just saw the pictures =D) Particularly on the page 12, scheme 17. Scheme 20 seens good too, because it has regioselectivity. Forgive me if I am missing something...

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