Re: [DIYbio] Can I do these tests at home? Parasites, chlamydia...

You couldn't rig up a 1080p webcam above the thermocycler and just have it take snapshots however often?

On Tuesday, June 14, 2016, Bryan Jones <bryanjjones@gmail.com> wrote:

That would be tricky. I'd be surprised if you could get the sensitivity & accuracy with a web cam and up light. But the bigger obstacle is that qPCR measures florescence between each of up to 40 cycles. So every couple minutes for a few hours you would have to pause your thermocycler, take out your samples, run to the dark room, take a picture, run back, put the samples back in the machine and unpause. Not impossible, but not exactly an efficient protocol.

On Tue, Jun 14, 2016, 4:09 PM CodeWarrior <code.w4rri0r@gmail.com> wrote:

Surely the only hard part of doing qPCR is getting the fluorescent agent? A standard webcam with a coloured filter over it and a uv light in a dark room should work no? Especially if you have a negative controle to measure background illumination against.

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