You're right that qPCR is usually outside the scope of DIY bio, although the Chai Bioscience Open qPCR does bring it a lot closer ($3000 for the base model). You can do qPCR without the special florescence instrument though, like you suggested it would involve running bands of DNA on a gel and comparing the intensity.
Traditional PCR would only test for presence/absence of the specific organism, it couldn't tell if that organism was the major cause of symptoms. That would be pretty useless if you are trying to test for a commensal organism that might be causing an opportunistic infection, but if you are healthy there should be zero giardia DNA in your urine, so I would think that a binary test should be sufficient.
Traditional PCR would only test for presence/absence of the specific organism, it couldn't tell if that organism was the major cause of symptoms. That would be pretty useless if you are trying to test for a commensal organism that might be causing an opportunistic infection, but if you are healthy there should be zero giardia DNA in your urine, so I would think that a binary test should be sufficient.
On Tue, Jun 14, 2016 at 1:08 PM Gordana Ostojic <gordana.n.ostojic@gmail.com> wrote:
I understand that, but equipment-wise it is more complicated. DIY community does traditional PCR, at least I noticed that here, people mostly do pcr, gel electrophoresis for detection/verification. Maybe if one is really confident with the protocol, one can estimate original DNA amount by the electrophoresis line intensity, like compare to a known amount, but overall sounds very difficult.--
On Tuesday, June 14, 2016 at 12:11:10 PM UTC-5, CodeWarrior wrote:qPCR doesn't just tell you what DNA you have but how much dna you have.
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