The most common dye used is sybr green, which absorbs in the blue spectrum and, as the name implies, emits in the green spectrum.
On Tue, Jun 14, 2016, 6:21 PM Gordana Ostojic <gordana.n.ostojic@gmail.com> wrote:
How are they removing intercalated dye during or after the cycle? Also dyes are typically prone to bleaching under constant illumination and stability can be messed up during the thermal cycling. UV also requires careful setup, lots of things absorb the UV, including DNA. They probably use some other dye for intercalating.--
On Tuesday, June 14, 2016 at 4:09:30 PM UTC-5, CodeWarrior wrote:Surely the only hard part of doing qPCR is getting the fluorescent agent? A standard webcam with a coloured filter over it and a uv light in a dark room should work no? Especially if you have a negative controle to measure background illumination against.
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