hey guys! I was able to culture some bioluminescent bacteria from squid and I want to know what it is. My guess is that its V. Phosphoreum but to confirm, I want to extract it's DNA and try the 16S rRNA identification with primers from Odin (http://www.the-odin.com/bacterial-16s-rdna-primers-for-bacterial-identification/). Should I be able to get PCR-able DNA out with dish detergent and ethanol/isopropanol? Any good protocols that are DIY friendly?
-- on a side note, does anyone have either a) good DIY gel purification method or b) someone that will sell kits to ronin-hackers (do not belong to any institution)?
as always, thanks!
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