Re: [DIYbio] Re: A hypothetical protocol for DIYBIO DNA synthesis

Hi all,

This is an awesome project. Kind of puzzled why I don't see much publications on the topic.
It's possible it has been already shifted to patent applications? (Found one using acid decapping meanwhile)

I saw pretty much a lot of ideas thrown out there, is there anyone that started testing? Ideas are running wild here, but about the basic experiments to validate the procedure?

Microfluidics seems the most straightforward approach, especially if you look for solid state synthesis. I'm thinking to give a try to the theory with the digital microfluidic device we've built at digi.bio. Using magnetic beads fuctionalized to oligonucleotides would allow programmable exposure of elongating chain to different reagents, concentrations and incubation times.

Something like elongate>wash>exonuclease>wash>decap>wash>elongate

If I make enough devices I can test probably 100x different reaction protocols and map the search space for optimal conditions.


@codewarrior I was discussing your proposal at the diybio meetup in amsterdam, I was pretty surprised to see so much involvement. We thought starting a community project there.

Best,
Fede

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