Re: [DIYbio] Biorad micropulser electroporator question

You were using an electroporation cuvette, right? (I can't tell)

Here is a protocol that worked well for me, with E.coli overnight
subcultures and 18MOhm water... your medium may have been too salty
(conductive):
https://groups.google.com/forum/#!msg/diybio/JcKyNIQGLIM/u4qIOllQRnAJ


Also, FYI:
Here are tables of voltage settings for various organisms...
http://m.imgur.com/a/swOXq

Also from this BioRad doc, pg 28
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006174B.pdf
(screenshot here too) http://imgur.com/TmwPOho
Most Interestingly: E.Coli 12-18kV/cm, test at 5msec pulse, adjust
from that starting point for efficiency tuning
S.cerevisieae ~7.5kV/cm, start at 5msec pulse

On Sat, Oct 29, 2016 at 6:59 AM, tom a. <jupm19@gmail.com> wrote:
> Does anyone have any experience with a Biorad MicroPulser Electroporator? I
> made sure that the metal prongs were touching the metal sides of the
> cuvette, but it still errored out. I've never used one before, and want to
> make sure I'm doing it right.
>
> Thanks in advance,
> Tom
>
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--
-Nathan

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