Re: [DIYbio] Anyone ever see electrophoresis where the gel was rolled?

On 03/16/2017 05:54 AM, Nathan McCorkle wrote:
> Maybe the
> unraveling-preventer could seal one end, while the other was upright
> and exposed to air, allowing you to then pour in running buffer.
> Anyway, I'm just thinking of unconventional ways to emulate capillary
> electrophoresis, mainly so I don't have to purchase a traditional gel
> box... but also thinking to reduce setup/cleanup time involved, and
> reducing reagent consumption.

I like the idea. Here are my thoughts:

Don't do a tight roll, but use a filler cylinder in the center so
rolling/unrolling is not too different radius/ curvature from inside to outside.

Is unrolling needed at all? If thinking of "the usual way" it seems to be needed so you can see
the gel -- and use the center of the moving blobs of molecules and reject fuzzy edges.
So, unrolling is probably needed until some near perfect shaped rolled channel can be made filled with gel
inexpensively -- perhaps 3D printing the insulating channel in a spiral would be good...

If rolling sheet material then running electrophoresis, the best sharpest part of bands in the middle could be cut out
by chopping through the whole thing with a guillotine like cigar cutter machine. Would that distort bands some?
Yes, if they were soft

Use two layers of insulator so unrolling goes without tearing chunks of gel out.

3D printing the insulating channel in a spiral:

After making a spiral channel, fill with gel and any buffer wanted, then wipe or squeegee to get top edges
dry. This could be done like litho printing and scale up. Next apply a film and bond it with pressure to seal in
gel and insulate. Next connect terminals for volts and current. Terminals might be part of the 3D channels, or maybe bits of
wire stabbed through top film in early tests. For terminals that are part of the 3D channels the film would cover them,
and next they would be uncovered by laser-cutting such that the terminal conductors don't cut through, but the film does get
blasted away to reveal a contact surface. The whole thing could be engineered to be translucent and image through it
to quantify electrophoresis -- either post-processed or as it moves.

John Griessen

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