Is there any worry that the amp resistant pKD46 plasmid will be extracted into the SLiCE lysate, in which case this plasmid will carry through to the final transformation? To me it would seem you can't use it to make amp resistant vectors..or maybe I'm missing something. Thanks for the clarification.
-- RC
On Friday, October 21, 2016 at 11:22:11 AM UTC-7, Bryan Jones wrote:
On Friday, October 21, 2016 at 11:22:11 AM UTC-7, Bryan Jones wrote:
In my most recent test, without pKD46, I got 10 colonies from the SLiCE reaction, which was the same as the no SLiCE control. SLiCE with pKD46 resulted in 50-60 colonies. For comparison, NEBuilder resulted in about 100.
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