Re: [DIYbio] Cheap capillary electrophoresis



On Tuesday, April 11, 2017 at 4:21:34 PM UTC-5, John Griessen wrote:
On 04/10/2017 01:23 AM, Harristotle wrote:
> Capillary electrophoresis seems like such a good fit for DIY bio: No gel media, cheap sybase dyes, good possibility for optical
> detection. I thought I would have a go, and this video is my report of my first efforts.

What is the capillary initially filled with, a conducting buffer solution?  No gel needed, just the narrow capillary path?

For DNA, it needs buffer soluble polymer as a sieve (pvp for example), matrix flows but slows everything. Companies use PA gel inside for high resolution throughput. That one is fixed.  

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