[DIYbio] A question about generating plant calluses

Dear plant DIYers,

I am gearing up towards a plant DIY operation, and I was hoping you could answer a couple of questions that I am having a hard time finding on the internet. 

 1.  Do calluses need light to grow? Some protocols grow them in the dark, so I am confused.  If so, what lights/cycle do you recommend? My purpose for for growing calluses (calli?) would be two-fold: somatic embryogenesis, and transformation with Agrobacter in Crassula. 
 2.  Can I use parafilm to seal the callus-growing petri dishes? What about gas exchange? 
 3. Would the PPM in the callus media kill Agrobacter?

many thanks in advance!

Patricia


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