Re: [DIYbio] Where do you get sequencing done

Amplicons can be difficult to sequence on new platforms. The massive parallel sequencing isn't designed to work with populations that have extremely similar sequences.

You may want to see if there is anyone out there providing pyrosequencing services. This method is better / more accurate for determining the sequence of a single gene or amplified product

On Thu, Oct 26, 2017 at 11:53 AM <djwrister@gmail.com> wrote:
Laragen has been cheap and convenient. Doubt if you need a business address since they send nothing to you. You download the sequencing results. 

On Oct 26, 2017, at 11:32 AM, Hans Wilms <hanstwilms@gmail.com> wrote:

Just a DNA amplicon. And thank you, Dan, I'll check it out!

On Thu, Oct 26, 2017 at 8:57 AM Skyler Gordon <skgor1@gmail.com> wrote:
You want to sequence a DNA ng  from a plasmid or you want to send them a whole plasmid and have it sequenced?

-SG
On Wed, Oct 25, 2017 at 8:36 AM <djwrister@gmail.com> wrote:
I use Laragen in Culver City, CA


Dan Wright

On Oct 25, 2017, at 8:01 AM, Hans Wilms <hanstwilms@gmail.com> wrote:

Hey everyone,
Anyone know of a good place in the US to send a plasmid for a quick Sanger sequencing? A place that will accept a sample sent from my residential address?
--
Hans T. Wilms
PhD student, GSU
Charlie Benson Lab

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