Re: [DIYbio] DIY electroporator

My main reason for reading this group is to help people avoid hurting themselves. Please think through this project. High voltage, especially high voltage with large capacitors, is very dangerous. I'd be happy to look over a design, but there are lots of ways to kill yourself with the components you are looking at.

> On Mar 28, 2018, at 12:26 PM, John Griessen <john@industromatic.com> wrote:
>
> On 03/28/2018 06:25 AM, nisha p wrote:
>> PS: what type of energy storage capacitors should i opt for metalized propylene or ceramic capacitors
>> On Wed, Mar 28, 2018 at 3:21 PM, nisha p <nisha.puthukudi@gmail.com <mailto:nisha.puthukudi@gmail.com>> wrote:
>>    i will measure resistance today.what should I add in this circuit to get pulses i require.
>>    thank you for your help
>
> I've pointed you to my open hardware project before.   These kinds of vague questions would be better asked on
> the list for that project rather than diybio.  The hardware details and many possible directions would bore many on this list.
>
> Here's a link to the list:  https://cibolo.us/mailman/listinfo/open_electroporator
>
> Culture shock is not really a DIY design, but maybe you can take parts and reuse for a super simple version
> using your 1500V transistor and a metal shield around it to block its high radio noise.
> I think the FCC rules even allow unintentional radiators that are intermittent firing.
>
> I have not gone in that direction because I am sure it would be more laborious to build
> and have higher material costs, so not good as a product.
>
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