Re: [DIYbio] Nanoporing a human genome

Hello,

I like your idea!

Oxford Nanopore (O.N.) long read technology is great for deciphering portions of the genome that would usually go unnoticed - such at short tandem repeats and homopolymers - and is also great for creating long contiguous sequences (or "contigs") that can then be used to align to one another more accurately.

That being said, the accuracy of base calling just isn't the same with the Nanopore read technology.

Today, most labs doing full genome sequencing are using O.N. to generate a skeleton genome map and then using Illumina sequencing to "polish" the map with more accurate sequence data.

There is a company in the United States (Texas) called "Mr. DNA". They sequence pretty much what ever you send them.

There are also numerous genomics sequencing "cores" at universities and institutes around the United States that supplement their research with funding from sequencing. UCSD and The Scripps Research Institute both have DNA sequencing cores that provide Illumina and Oxford Nanopore technologies.

-SG

On Tue, Apr 3, 2018 at 2:22 PM Andreas "Mega" Stuermer <andreas.t.stuermer@gmail.com> wrote:
Hi!

We are going to sequence a human genome with a nanopore in the Ars Electronica. We want to compare this to the genome sequence that a commercial company provides us with.

Any input which company offers full genome sequencing for a low price? Highly appreciated

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/b9554801-abbe-4d36-93c9-233b42119bc2%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAGA%2B4LX7ZhkbtNLac6L%3D90n2Fm6n18rJCf7RQd3G8Fo3hvPWMA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment