[DIYbio] General mtDNA extraction difficulty

My main experience so far has been with bacterial genetics. Given bacteria are the workhorses of vinegar fermentation the intimate details of this and related analysis and sequencing have been focused around these. I am working on a DIY Bio guide and maybe even physical kit to let people use raw vinegar from the grocery store to culture acetic acid bacteria (completely non-pathogenic), extract chromosomal DNA, PCR 16S rRNA (or several DNA housekeeping genes for a tighter match) and BLAST the results.

I got thinking about another, probably more widely interesting, kit that I am not sure is feasible. In short, it would be a kit to extract mtDNA from animal or fungal cells (not sure if you need chitinase to deal with the latter) and sequence conserved regions of cytochrome c to identify what exactly you have sampled. I know this will be a lot harder, noticeably extracting the mtDNA not just the nuclear DNA, and probably all kinds of limited availability reagents and even equipment that will add bumps on the road but is this even something feasible outside of someone like the few of us that have lab spaces and equipment? Also I am unsure if the nuclear DNA and mtDNA needs to be separated by centrifuging or chemical means so as not to screw up the PCR. Cytochrome c seems to be pretty reliable as an identifier at least at the genus level, correct? Thoughts?

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