Re: [DIYbio] Opentrons OT-2 release

Hello, 

I am writing to you because we are currently on the verge of buying on Opentron OT-2. One of the main purposes is making DNA mix for MoClo assembly. I have seen on your message that you are working on adding colonies picking feature to the OT-2. How is the work going? Is it an opensource work? Do you need some help? 

I am a Ph.D. candidate from France in molecular biology.  I have relatives that are very good developers, so maybe we can help a little. 

Looking forward to hearing from you soon,
Victor 





Le mardi 27 mars 2018 20:55:38 UTC+2, Koeng a écrit :
Right now, we are working on a colony picker (FreeGenes project). It's been going slowly since we've been jamming on some database organization stuff. In combination with the Bionet project, we have a system in the works called OpenFoundry which should allow completely automated assembly, transformation, plating, and picking of DNA for MoClo assembly. Everything actually works so far on the bot except the picking. To solve that, we got a the XL transilluminator box from IO rodeo, and cut out some acrylic that should be able to perfectly fit a 96 well plate. Since we do everything on Nunc rectangular plates which are the standard size, we should be able to attach a webcam to the top of the box to get really nice clear and standard pictures of the colonies. Then port that data through OpenCFU, and convert image coordinates to OpenTrons coordinates. Pick with pipette tips.

Anyway, the robots really work well for automated cloning. The multichannel pipettes are also quite nice, and this one is about 2x faster than the sped up versions of the OT-1 (we have one in lab)

Koeng

On Tuesday, March 27, 2018 at 11:29:12 AM UTC-7, Dakota Hamill wrote:
I think OpenTrons people are on this list.  Followed them with interest for quite sometime.

If you're on here, any colony pickers in the works?  Or 8 span heads? 

On Tue, Mar 27, 2018, 1:31 PM Koeng <koen...@gmail.com> wrote:
Hey all, 

Saw OT-2 was released from OpenTrons http://opentrons.com/

The new robot is a lot faster and more polished than the previous version. The 'open' aspect of the software is really useful - you can make scripts to do almost anything! 

Koeng

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