Re: [DIYbio] Opentrons OT-2 release

Yep, we leave the plates on the opentrons for drying. 

I think I might have accidentally responded incorrectly: The OpenTrons doesn't aliquot the competent cells. I do that myself in the cold room because we generally don't want to put a robot in there. Also, electronic pipettes make it very easy. However, in that manual pipetting, I didn't mix, which I presume is a major problem. 

For me, the #1 add on to an OpenTrons would be a plate handler + a microplate sealer. If I had that, I could automate nearly everything on the machine. (except making mastermix, because I don't trust the bots to get every last drop of enzyme)

After vortexing, there is a lot of liquid on the microplate seal. When I tear it off, liquid gets everywhere and it sucks because it can cause contamination and majorly reduces the liquid in each well. An improv plate spinner made of a salad spinner fixes all that! Though, now that you mention it, might be a good way to get liquid out of plates after cleaning them.

Koeng

On Monday, June 25, 2018 at 12:53:05 PM UTC-7, John Griessen wrote:
On 06/25/2018 12:46 PM, Koeng wrote:
> On 06/25/2018 11:30 AM, John Griessen wrote:
> >Sounds really good.  Does your tray need a mini shaker to stay ready longer?
> The trays/rectangular plates usually need to dry a LOT to soak up the serial dilutions quickly.

So, that means you leave the plates in the opentrons drying for long periods of time?
If so, it does seems like shaking/circular motion would be good for recovering from your case of
"Don't let competent cells settle at the bottom of the trough"?
Maybe forced dessicating by stirred air is desirable too?
This all starts me thinking of a conveyor for trays and a tray stacker at one end.

> For the actual synthesis plates, I
> just sort of vortex them and then put them in a salad spinner to spin the liquid to the bottom (this works FANTASTIC: Better than
> what I would ask from any piece of actual lab equipment and it's only like $15).

So, does this mean that the salad spinner is a way to get all the liquid out of the trays and all mixed together
in the bottom of the spinner?

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