[DIYbio] Re: Tissue culturing any plant

Had to edit for clarity 



On Friday, July 27, 2018 at 2:14:45 PM UTC+2, Andreas "Mega" Stuermer wrote:
Hi guys, 

after routinely culturing tobacco for some time, we have been trying some other plants out of the woods. Attached is a short description how to do the hormone assay. Basically it should be possible to clone any given plant on MS medium (ingredients listed) but it is crucial to get the concentrations right. 

I do have to say that the sterilization process of 30 seconds in Ethanol and 3 minutes in 35% household bleach seemed too weak - most of the petri dishes still molded, which isn't the case for inside-grown tobacco.


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