Re: [DIYbio] was: glowing plant - Open Source chloroplast engineering

I have seed from my gleaux plant, a bunch that dropped into the pot
that it was planted in have sprouted so I know they're viable. I never
saw the original glow, nor these... and they don't show up with a 30
second dSLR exposure image. I had been working to setup my laptop to
remotely trigger my camera to take a series of images and then add a
bunch of images together to try stack the brightness... but I got
caught up with other things and then my laptop went through 3 weeks of
failed ubuntu upgrade attempts before I got it straightened out again
recently.

Anyway, if you think you could use some seed, how many would you want?
On Fri, Oct 26, 2018 at 5:57 PM Andreas "Mega" Stuermer
<andreas.t.stuermer@gmail.com> wrote:
>
> Hi guys,
>
> I am currently re-visiting the idea of chloroplast engineering. Chloroplast engineering yields loads of proteins, and is only maternally inherited (containment bonus). Antibiotic selection marker will be removable with Cre-Lox, and should happen with low frequency by iself too.
>
> First project: creating the empty vector, SciHouse in Florida will store the vector in bulk and share with anyone interested (open source)
> Second project: putting the lux operon in, just because. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0015461 *
> I have seen one of these plants, and they were visibly glowing to the naked eye (but weak). Note that that was way before the Silicon Valley glowing plant cowboy project.
>
> *Unfortunately they stopped selling them in the United States for no apparent reason (maybe they didnt't get enough customers).
>
> Not sure if I should start with weeping willow or tobacco. I've been routinely working with tobacco and can share my protocols with our US collaborators so they can do the tissue culture there. Tobacco is pretty lame though.
>
> Anyways, just wanted to see if anyone would be willing to chime in, synthesis is expected to cost around 500$. Attached the file. Addition of lux will just be one PCR step
>
>
>
>
> --
> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
> To post to this group, send email to diybio@googlegroups.com.
> Visit this group at https://groups.google.com/group/diybio.
> To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/a90a14a1-7960-415a-976d-2ce034fcaa74%40googlegroups.com.
> For more options, visit https://groups.google.com/d/optout.



--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9JtQ5eXYE_7sJYsfZ0jVaLMmvLrwPh52dve%3D3K7pgcJhA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment