Re: [DIYbio] was: glowing plant - Open Source chloroplast engineering

Gene gun has the advantage that you can shoot it into leaf tissue and then regenerate it. 

Electroporation of protoplasts may be possible but they are easy to burst

On Sat, Oct 27, 2018, 10:25 John Griessen <john@industromatic.com> wrote:
On 10/26/18 7:57 PM, Andreas "Mega" Stuermer wrote:
> First project: creating the empty vector, SciHouse in Florida will store the vector in bulk and share with anyone interested (open
> source)

How do you do transformation?  Is electroporation used?  I'd like more beta testers of my electroporator.
So far, the folks that have gotten one have never used it on live cells.  Kind of slows the project momentum...

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