Hey Derek,
So I did a mental walk through of doing some RT PCR sort of testing but there are many big question for me.
- what value does this give? The two reasons for testing is to identify it for treatment and containment. Community labs dont have the ability to treat or training in dealing with people with positive infections who might come in so it could make them a place highly likely to put the community lab members at risk, for epidemiology its needed to have central tracking so where does this data go to?
- are the primers any good? I know the WHO tests seem to the better ones, the CDC initial test was a multi disease test that didnt work right so they are in the midst of switching systems, and need to centralize where the data is kept for tracking of outbreaks.
- What are the possibilities for safety considerations?
Overall I want to find ways for the DIY bio community to serve their communities during such times but I also want to make sure we keep ourselves and communities safe.
Thoughts on this?
On Fri, Feb 28, 2020 at 9:19 AM Derek <derekja@gmail.com> wrote:
Hey Tito,
Been doing some research on this. With the long incubation time I'd like to know when it's circulating in the community before cases are seen. Have found an rt-pcr protocol and located a company that will sell me a plasmid with part of the capsid envelope to use as a positive control. Biobasic has all the primers cheaply. Still working on a collection protocol to allow me to swab banisters, etc. A bunch of folks at the victoria makerspace are interested in helping so will eventually be documenting our steps. Happy to share any details.
Derek
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