Re: [DIYbio] DIY COVID-19 PCR test

I work in the industry as a surplus bio/genetic equipment supplier. I was checking out the CDC recommendations and kits available for corona virus identification and it looks like older ABI equipment, such as the 9700 silver and gold thermal cyclers and the ABI 7500 Fast Analyzers are going to be in demand for this testing. It's funny because these are practically antiques now, and supply is very limited. I don't see much progress towards a vaccine until late this year... so be ready; there will be quite a demand in detection of this new pandemic.        Jerry, CSI Sonora California

On Fri, Feb 28, 2020 at 9:31 AM William Heath <wgheath@gmail.com> wrote:
I am interested.  I am a software engineer.  How can I contribute/help in this process?

-Tim BCSE, MSCS, MBA

On Fri, Feb 28, 2020 at 12:19 PM Derek <derekja@gmail.com> wrote:
Hey Tito,

Been doing some research on this. With the long incubation time I'd like to know when it's circulating in the community before cases are seen. Have found an rt-pcr protocol and located a company that will sell me a plasmid with part of the capsid envelope to use as a positive control. Biobasic has all the primers cheaply. Still working on a collection protocol to allow me to swab banisters, etc. A bunch of folks at the victoria makerspace are interested in helping so will eventually be documenting our steps. Happy to share any details.

Derek

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