Hi Tom De Mets,
Here is the website where I've bought it: http://gaudi.ch/PocketPCR/ We'll see if it works...
About mineral oil, I don't know. I image that the oil should not have rest of DNA, proteases, DNases, or other molecules that can affect the PCR.
Firstly I will try without oil. Somebody in this post said that if your sample is big enough I will not need the oil.
El sábado, 5 de septiembre de 2020 a las 5:57:03 UTC+2, Tom De Medts escribió:
Hi NicVied,Can you please share a link to this pocker PCR, thank you.Are you going to later mineral oil in lieu of a heated lid?If yes, then will you buy it from a scientific lab supplier or just from some marketplace like amazon?I found this link for the least expensive mineral oil - https://www.amazon.com/Mineral-Pure-Organic-Ingredients-Utensils/dp/B07G5KVW4G/ref=sr_1_33?dchild=1&keywords=mineral+oil+PCR&qid=1599277973&sr=8-33but I am not at all sure if this will serve the purpose...I look forward to your response.Cheers!
TdMOn Fri, Sep 4, 2020 at 3:36 AM NicVied <antoni....@gmail.com> wrote:Thanks you all, finally I bought the pocket PCR. It's really cheap but without "hot lid", old school style. I will let you know how it work!El miércoles, 19 de agosto de 2020 a las 4:36:51 UTC+2, jfo...@udel.edu escribió:It depends to some extent on how "diy" you want to be and what your skill set is... I cobbled together a serviceable thermocycler that can do a half dozen epi tubes from the cooling block from an old HP workstation I had in my attic, a cartridge heater, an arduino w/ thermocouple, and some other electronic bits and pieces I had lying around. Material cost was basically nothing, but for the time I spent tinkering to make it serviceable I could have picked up a consulting gig and made enough money to buy a nice, shiny, new one...---- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
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