> Independent of the hardware, how do you propose to append promoters, markers, tails, etc into the gene(s) desired ? Getting them expressed may be non-trivial...
GoldenGate. Expression is outside of outside of scope, though it's kinda easy in my experience.
> Is there a Q.C. step that's understandable for intermediate results ?
Sequencing.
> Being specific to answer this may benefit you... Answering a question to others often clarifies it for yourself too.
I've cloned thousands of genes myself and was part of designing of many genetic toolkits (led https://stanford.freegenes.org/ for 3 years). Also a main contributor of Poly (https://github.com/timothystiles/poly), and founded a company doing full automation for synbio (in particular, the DNA assembly space). I've built functioning systems for this task before, which is honestly quite a bit more illuminating than talking about building them.
On Friday, August 19, 2022 at 7:33:49 PM UTC-7 dank...@gmail.com wrote:
Hello 'k-101',
Independent of the hardware, how do you propose to append promoters, markers, tails, etc into the gene(s) desired ? Getting them expressed may be non-trivial...
Is there a Q.C. step that's understandable for intermediate results ?
Being specific to answer this may benefit you... Answering a question to others often clarifies it for yourself too.
ex: a Fat jupyter notebook full of files and notes, or what ?
Big projects are fun projects, you named yourself one.
Good luck !
my ref: 19 Aug 2022, DIYbio, NAFL, koeng101, Question about BP build out
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