Re: [DIYbio] DIY Refrigerated Centrifuge

Do be careful if deciding to cool the entire centrifuge. The centrifuges that have built-in cooling generally have at least some of the electronics encased in conformal coating to prevent issues with condensation. The small tabletop centrifuges I've taken apart don't have this feature. If your lab happens to have a dry air source (e.g. for IR spectrometry) then you could maybe do something with that to prevent condensation. If you are cooling the centrifuge itself then you likely want to add condensation protection and I don't recommend manually adding conformal coating to the circuit boards without also adding extra cooling for the mosfets since the mosfets in the three-phase inverter dissipate enough power that overheating is a common failure mode in the medium format eppendorf refrigerated centrifuges even though those are strapped to a block of aluminum which is then strapped to the thick steel case of the centrifuge chamber. Normally I'd be gung ho about people hacking their gear and seeing what breaks but I assume you don't want to risk killing your centrifuge with the difficulty of importing stuff into Sri Lanka right now.

On Sun, Oct 23, 2022 at 5:12 PM Jonathan Ferooz <atgs...@gmail.com> wrote:

Putting the centrifuge in a cool bag with cool pack? Or just putting the rotor in a fridge before?

Le 22/10/2022 à 19:59, Kavish Sirisena a écrit :

Greetings fellow researchers! 

I am in need of a refrigerated centrifuge to process some proteins samples extracted from a mammalian cell line. The lab that I currently work does not have a refrigerated centrifuge to accommodate this. Is there any other alternative way to reduce the temperature of the working conditions?

I was thinkin maybe I could suspend a small eppendorf tube inside a bigger tube filled with water and subject it to freezing. However I am not sure how this would affect the centrifugation procedure.

Looking for your valued opinions on this matter!

TIA

Kavish
IRD Genetics - Sri Lanka

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