Re: [DIYbio] Re: Is BioBricks Free Genes Project still maintained and alive?

Yeah I still operate in this area. Took like 2-3 years off though to go cofound a different company in robotics, but back at it.

Nowadays I'm making some open source part toolkits from oligo pools (got a quite good oligo assembly method) myself. Synthesis is kinda cheap if you go to pools. These are my latest designs I designed over a couple days:

Roughly 175 parts, but definitely gonna be making more soon.

.
├── bsub
│   ├── BsubPars.md
│   ├── DS_amyE.md
│   ├── DS_glmS.md
│   ├── DS_lacA.md
│   ├── DS_sigF.md
│   ├── DS_thrC.md
│   ├── DS_yhgE.md
│   ├── DS_yrbD.md
│   ├── P3P.md
│   ├── P43.md
│   ├── P_J23101.md
│   ├── PlepA.md
│   ├── PliaG.md
│   ├── PliaI.md
│   ├── Pman_manR.md
│   ├── Pmtl-short.md
│   ├── Ptet_tetR.md
│   ├── Pveg.md
│   ├── PxylA.md
│   ├── PxylA_xylR.md
│   ├── TO_pE194ts.md
│   ├── TO_pHT315.md
│   ├── US_amyE.md
│   ├── US_glmS.md
│   ├── US_lacA.md
│   ├── US_sigF.md
│   ├── US_thrC.md
│   ├── US_yhgE.md
│   ├── US_yrbD.md
│   ├── camR-Bsub.md
│   ├── eryR-Bsub.md
│   ├── kanR-Bsub.md
│   ├── pAMbeta1.md
│   ├── pBM300.md
│   ├── pBS72.md
│   ├── pCG1.md
│   ├── pCM66.md
│   ├── pGRB1.md
│   ├── pIP404.md
│   ├── pLS20.md
│   ├── pMK3.md
│   ├── pMTLB.md
│   ├── pMflST.md
│   ├── pNG168.md
│   ├── pSK41.md
│   └── spcR-Bsub.md
├── kmax
│   ├── ARS1.md
│   ├── ISceI_Kmax.md
│   ├── OpenCRISPR_yeast.md
│   ├── PARS1.md
│   ├── PIN450v2_promoter.md
│   └── ntag_INU1.md
├── mtk
│   ├── MTK2_001.backup
│   ├── MTK2_003.backup
│   ├── MTK2_004.md
│   ├── MTK2_005.md
│   ├── MTK2_006.md
│   ├── MTK2_007.md
│   ├── MTK2_008.backup
│   ├── MTK2_009.md
│   ├── MTK2_012.md
│   ├── MTK2_013.md
│   ├── MTK2_014.backup
│   ├── MTK2_015.md
│   ├── MTK2_016.md
│   ├── MTK2_017.md
│   ├── MTK2_018.md
│   ├── MTK2_019.md
│   ├── MTK2_020.md
│   ├── MTK2_021.md
│   ├── MTK2_022.md
│   ├── MTK2_023.md
│   ├── MTK2_024.md
│   ├── MTK2_025.md
│   ├── MTK2_026.md
│   └── blank.md
├── other
│   └── green_smutans.md
├── pichia
│   ├── EGFP_Pp.md
│   ├── PARS.md
│   ├── Pichia_Bxb1.md
│   ├── RFP_Pp.md
│   ├── enzymes
│   │   ├── BbsIA_Pp.md
│   │   ├── BbsIB_Pp.md
│   │   ├── BsaI_Pp.md
│   │   ├── BtgZI_Pp.md
│   │   ├── Esp3I_Pp.md
│   │   ├── GFP11_Pp.md
│   │   ├── GFP1_10_Pp.md
│   │   ├── OpenTn5_Pp.md
│   │   ├── PNK_Pp.md
│   │   ├── PfuSso7d_Pp.md
│   │   ├── RKOD_Pp.md
│   │   ├── T5exo_Pp.md
│   │   ├── T7RNAp_Pp.md
│   │   ├── T7endoI_Pp.md
│   │   ├── TaqLigase_Pp.md
│   │   ├── TaqPolymerase_Pp.md
│   │   ├── phi29_Pp.md
│   │   └── phiBT1int_Pp.md
│   ├── ntag1_2
│   │   ├── CtUlp1_Ec.md
│   │   ├── ntag1_pichia_alphaAmylase_alphaMFDelta.md
│   │   ├── ntag1_pichia_alphaMF.md
│   │   ├── ntag1_pichia_alphaMFDelta.md
│   │   ├── ntag1_pichia_alphaMFDelta_noKex.md
│   │   ├── ntag1_pichia_alphaMF_noEAEA.md
│   │   ├── ntag1_pichia_glucoamylase_alphaMFDelta.md
│   │   ├── ntag1_pichia_inulinase_alphaMFDelta.md
│   │   ├── ntag1_pichia_invertase_alphaMFDelta.md
│   │   ├── ntag1_pichia_killer_alphaMFDelta.md
│   │   ├── ntag1_pichia_serumalbumin_alphaMFDelta.md
│   │   ├── ntag2_gfp11_cbm3_sumo.md
│   │   └── ntag2_mCherry_sumo.md
│   ├── ntag_pichia_alphaAmylase_alphaMFDelta.md
│   ├── ntag_pichia_alphaMF.md
│   ├── ntag_pichia_alphaMFDelta.md
│   ├── ntag_pichia_alphaMFDelta_noKex.md
│   ├── ntag_pichia_alphaMF_noEAEA.md
│   ├── ntag_pichia_glucoamylase_alphaMFDelta.md
│   ├── ntag_pichia_inulinase_alphaMFDelta.md
│   ├── ntag_pichia_invertase_alphaMFDelta.md
│   ├── ntag_pichia_killer_alphaMFDelta.md
│   ├── ntag_pichia_serumalbumin_alphaMFDelta.md
│   ├── pAOX1_promoter.md
│   ├── pENO1_promoter.md
│   ├── pTPI1_promoter.md
│   ├── tAOX1_terminator.md
│   └── tAOX1_terminator_ctag.md
└── yeast
    ├── 2micron.md
    ├── ALD6_promoter.md
    ├── CCW12_promoter.md
    ├── CEN_ARS.md
    ├── CUP1_promoter.md
    ├── GAL1_promoter.md
    ├── HHF1_promoter.md
    ├── HHF2_promoter.md
    ├── HIS3.md
    ├── HTB2_promoter.md
    ├── HygromycinR.md
    ├── KanamycinR.md
    ├── LEU2.md
    ├── MFA1_promoter.md
    ├── MFalpha2_promoter.md
    ├── NourseothricinR.md
    ├── PAB1_promoter.md
    ├── PGK1_promoter.md
    ├── POP6_promoter.md
    ├── PSP2_promoter.md
    ├── RAD27_promoter.md
    ├── RET2_promoter.md
    ├── REV1_promoter.md
    ├── RNR1_promoter.md
    ├── RNR2_promoter.md
    ├── RPL18B_promoter.md
    ├── SAC6_promoter.md
    ├── SceI_Scere.md
    ├── TDH3_promoter.md
    ├── TEF1_promoter.md
    ├── TEF2_promoter.md
    ├── URA3.md
    ├── Venus_Scere.md
    ├── Venus_Scere_GRAStag.md
    ├── ZeocinR.md
    ├── mRuby_Scere.md
    ├── mRuby_Scere_GRAStag.md
    ├── mTurquoise2_Scere.md
    ├── mTurquoise2_Scere_GRAStag.md
    ├── tADH1_terminator.md
    ├── tADH1_terminator_ctag.md
    ├── tENO1_terminator.md
    ├── tENO1_terminator_ctag.md
    ├── tENO2_terminator.md
    ├── tENO2_terminator_ctag.md
    ├── tPGK1_terminator.md
    ├── tPGK1_terminator_ctag.md
    ├── tSSA1_terminator.md
    ├── tSSA1_terminator_ctag.md
    ├── tTDH1_terminator.md
    └── tTDH1_terminator_ctag.md

On Thu, Jul 3, 2025 at 12:48 PM openbioscience <openbio...@gmail.com> wrote:

Last time I checked there was a message that FreeGenes deliveries were on hold due to resource unavailability.
Pitty. 

Keoni Gandall carried the torch for a while but I'm not sure if he still operates in this area because I have not heard about his recent activities.
I have some of the constructs but I had some issues with the fridge so I'm not sure if they are still viable.

Cheers,
Adrian
https://ottawabioscience.github.io/

On Thu, Jul 3, 2025 at 10:38 AM Koeng <koen...@gmail.com> wrote:

it dead I guess

On Thursday, July 3, 2025 at 4:31:46 AM UTC-5 Dorif wrote:

Hey, guys, what's happening with project site? It just redirects at some Turkish online game shop now. What the hell is going on there???

четверг, 19 января 2023 г. в 18:57:28 UTC+2, dank...@gmail.com:

The book "DIY Scientists Hack the Software of Life" is breezy and full of DIY stories not much technology or science really.

2011 Marcus Wohlsen

Well written from an entertainment point of view. but lots of the little side projects and people stories are interesting enough. I'd say worth a lib lend for sure. I happily Borrowed a paper copy from Toronto's bizarrely well equipt public library.


Lots of San Fran, NYC and Boston in this book of course.

And so it goes,

 

Daniel B. Kolis

my ref: Jan 2023,  https://groups.google.com/g/diybio/c/X7S8oFe1CPc. nafl, DIY

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