Re: how do you guys determine concentrations from spectrophotometry

On Wed, Nov 30, 2011 at 1:28 PM, Simon Quellen Field <sfield@scitoys.com> wrote:
> That was basically the method I was describing earlier, when someone
> complained that they could not get reference solutions of DNA.
>
> The other way is to use the Beer-Lambert Law directly, using molar
> absorptivity
> tables for the compound in the sample, and the absolute absorbance of
> the sample at a particular wavelength. The latter is the percentage
> absorbance
> using a null sample as 0%.
>
>
Ah, that's what I assumed everyone used. I think the problem with the
direct Beer-Lambert approach is the absorptivity. I never used that
method though I think chemists might use that method. You guys just
use excel also? Any other programs?

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