I was taught to use beer-lambert and standard concentrations with
extrapolation... beer-lambert in my biotech classes, extrapolation in
chem classes
On Wed, Nov 30, 2011 at 2:17 PM, Jeswin <phillyj101@gmail.com> wrote:
> On Wed, Nov 30, 2011 at 1:28 PM, Simon Quellen Field <sfield@scitoys.com> wrote:
>> That was basically the method I was describing earlier, when someone
>> complained that they could not get reference solutions of DNA.
>>
>> The other way is to use the Beer-Lambert Law directly, using molar
>> absorptivity
>> tables for the compound in the sample, and the absolute absorbance of
>> the sample at a particular wavelength. The latter is the percentage
>> absorbance
>> using a null sample as 0%.
>>
>>
> Ah, that's what I assumed everyone used. I think the problem with the
> direct Beer-Lambert approach is the absorptivity. I never used that
> method though I think chemists might use that method. You guys just
> use excel also? Any other programs?
>
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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