Re: Ultrasonic Misters -> Sonication?

To date though, has anyone attempted sonoporation or sonication using
those or any other form of consumer ultrasound? Were the results
negative or positive or inconclusive?

Sometimes I think we suffer the same problem as conventional
institutional science when it comes to failures; nobody reports
failures, so we end up repeating and repeating the same approaches until
it either works or becomes informal common knowledge that it doesn't.

I made a curtailed, half-assed attempt at sonicating some oil and water
using a mist-forming sonicator yesterday, and it didn't seem to work
very well. So, there's my failure report. However, I didn't get a fair
chance to try it out, as I was interrupted. Also, it made a huge mess;
as John pointed out I think, these things are designed to sputter water
everywhere!

On 19/01/12 11:19, Patrik wrote:
> There's also the ultrasonic jewelry and eyeglass cleaners. Typical
> models seem to be 42kHz, 35W, 1 pint (600ml) capacity.
>
> On Jan 18, 1:41 pm, John Griessen <j...@industromatic.com> wrote:
>> On 01/18/2012 10:24 AM, Cathal Garvey wrote:
>>
>>> What frequencies do these things operate at?
>>
>> 40 KHz to 80KHz
>>
>> I imagine the wattage is
>>
>>> going to be pretty low on these?
>>
>> 3 to 10 Watts often
>>
>> What frequencies and wattages do you
>>
>>> *need* to get stuff done like:
>>> - Electroporation?
>>> - DNA sonication?
>>> - Cell disruption?
>>
>> I'm sure you can get a small sample to do whatever cavitating sonic waves
>> will do, and at the small driver wattages found in those mister gadgets.
>> The drivers for misting are a little different shape than for waves, but
>> close to the same thing. They probably make a ring focused beam that is
>> aimed at the surface of the water reservoir so the surface is disrupted
>> into mist, where the usual setup is to make waves that travel and hit
>> tank walls or what is in the tank.
>>
>>> There's usually a water-level sensor on top to prevent them running
>>> while dry, which would break the unit, but you could always put it into
>>> a sample until entirely wet (without bubbles on the electret), turn
>>> upside-down at the surface, and then plug in?
>>
>> Sounds reasonable. OTOH, I got some piezo discs for cheap on ebay with experiments
>> in driving them as sonicators in mind. Think 0.05 USD.
>

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