[DIYbio] Re: Transformation questions

It would surely be worth a try!
25kV/cm for E.coli?

But isn't it better if I have a successful standard-transformation
done before trying something more difficult??

On 10 Mrz., 15:00, Nathan McCorkle <nmz...@gmail.com> wrote:
> Mega,
> you should just try to add E.coli from a plate to sterile water in a
> microtube. Add your DNA, then place the electrodes from a piezo
> sparker in and spark 1-3 times (or have 3 tubes, and try 1 spark on
> tube #1, 2 sparks on tube #2, 3 sparks on tube #3). Then add some LB,
> and incubate for 1 hr... then plate that on LB + amp plates.
>
> No CaCl2 or anything... its an experiment, but you could be the first
> to actually try it! (we've talked about it a lot on here, but never
> tried it)
>
>
>
>
>
>
>
>
>
> On Sat, Mar 10, 2012 at 8:19 AM, Mega <masterstorm...@gmail.com> wrote:
> > @
> > Chris:
>
> > That's amazing!!!
>
> > Did I understand that correctly?  Usually you transform roughly 1
> > cell  and 999 stay untransformed. Using this technique, ~600 out of
> > 1000 cells are transformed???
>
> > If that is true, I must ask a friend of mine (electrical engineer)
> > if he is interested to build such a miracle device!!!!!
>
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> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics

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