On Sat, Mar 10, 2012 at 9:00 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
> Mega,
> you should just try to add E.coli from a plate to sterile water in a
> microtube. Add your DNA, then place the electrodes from a piezo
> sparker in and spark 1-3 times (or have 3 tubes, and try 1 spark on
> tube #1, 2 sparks on tube #2, 3 sparks on tube #3). Then add some LB,
> and incubate for 1 hr... then plate that on LB + amp plates.
What kind of E coli is this? Non-competent E coli? Where do you get
that (I'm not sure what to search for)?
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