Re: [DIYbio] is it possible to isolate a unknown compound through HPLC

On Mon, Mar 5, 2012 at 6:46 AM, debadutta bhoi <debadutta1988@gmail.com> wrote:
> i have a crude extract of some plants if i want to know what kinds of
> compounds are there, is it possible to isolate each compounds from the
> crude extract through HPLC ?
> please help
>

Isolating the compounds is pretty easy, using HPLC, TLC, GC, etc...
For HPLC you just run your sample through the system, and at the end
of the line there is a spectrometer, when the signal changes the
liquid get diverted into a clean test tube, every time the signal
changes, a new test tube is brought into place to accept this
'fraction'.... I'm inclined to call this 1D (1 dimensional)
chromatography, because the separation of compounds depends on the
solvent/separation system used.... so you could in fact have multiple
compounds in each fraction. To separate those from each other, you'd
have to run each fraction through a different separation system than
the first one was (a la 2D gel electrophoresis).

Identifying these compounds is either expensive, or potentially a PhD
thesis sized project... but certainly do-able.

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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