I would use a UV-C light source. I have a hood at home that uses a UV lamp to sterilize glassware and cultures. On the coconut cultures, I think that you should be fine with autoclaving, this article says that it should work. (Scroll to pg. 122)
On Friday, May 18, 2012 8:04:49 AM UTC-4, Mega wrote:
Hey.--
I'd like to try to make a tissue culture.
According to this http://www.biologie.uni-hamburg.de/b-online/d29/29a. htm
The first tissue cultures were done using nutirent agar and coconut milk (for plant hormones).
So what I intend to do:
Mix LB-agar powder with coconut milk. Then autoclave. BUT: Do the phyto-hormones survive autoclaving?
Or should I just put it in the microwave and hope all bacteria are killed??
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