Sorry, I did not complete that.
Two discussion groups that would be useful are the hometissueculture group ( http://tech.groups.yahoo.com/group/hometissueculture/ ) and the Plant-tc List Serve at http://plant-tc.cfans.umn.edu/listserv/listsrvh.htm
-- Two discussion groups that would be useful are the hometissueculture group ( http://tech.groups.yahoo.com/group/hometissueculture/ ) and the Plant-tc List Serve at http://plant-tc.cfans.umn.edu/listserv/listsrvh.htm
You could do much better than LB-agar powder + coconut milk by using some of the simple basal media and synthetic plant growth regulators like the cytokinins-Benzyl adenine and Kinetin or auxins like IBA, 2,4-D.
Yes, microwaving is a good DIY option but autoclaving is much better. Autoclaving will destroy only some of the rarely used hormones and not the commonly used ones.
Murali
On 18 May 2012 22:07, Muralidharan E.M. <emmurali@gmail.com> wrote:
Two discussion groups that would be useful are the hometissueculture http://tech.groups.yahoo.com/group/hometissueculture/ and the Plant-tc List ServeOn 18 May 2012 17:34, Mega <masterstorm123@gmail.com> wrote:Hey.
I'd like to try to make a tissue culture.
According to this http://www.biologie.uni-hamburg.de/b-online/d29/29a.htm
The first tissue cultures were done using nutirent agar and coconut milk (for plant hormones).
So what I intend to do:
Mix LB-agar powder with coconut milk. Then autoclave. BUT: Do the phyto-hormones survive autoclaving?
Or should I just put it in the microwave and hope all bacteria are killed??
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